Studies on the preparation of docetaxel nano-liposomes and its treatment on liver cancer cells in vivo and in vitro
- VernacularTitle:多西紫杉醇纳米脂质体的制备及其对肝癌细胞的体内外治疗研究
- Author:
Youlong MA
;
Haiyan QI
;
Zhendong CAO
;
Dawei HU
- Publication Type:Journal Article
- Keywords:
docetaxel;
nanoliposome;
film-ultrasonic dispersion method;
HepG2 cell;
tumor therapy;
apoptosis
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(6):43-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepared the docetaxel nano liposome (L-DOC) for the therapy of liver cancer HepG2 cells in vitro and in vivo. Methods The film-ultrasonic dispersion method was used to prepare the L-DOC.The diameter and Zeta potential of L-DOC were determined by Nanosizer and the encapsulation efficiency was further measured.CCK-8 method was used to determine the cell viability of HepG2 cell after treating with various concentration of DOC and L-DOC respectively and the cell death type was detected by Flow cytometer.Next, we have studied the relative tumor volume change of tumor-bearing mice and the toxicity in vivo.Results The average diameter of L-DOC was 104 nm and the Zeta potential was about -35.1 mV.The Zeta potential of L-DOC was almost unchanged after standing for 96 hours.The encapsulation efficiency of L-DOC was ( 71.2 ±1.6 )%.The CCK-8 results showed that the cell viability was decreased after treating with various concentration of DOC and L-DOC, but the inhibition effect of L-DOC was better than that of DOC after treating with the same dose, especially for 20μg/mL.It was found that the cell death was induced by apoptosis.The in vivo study results showed that 6mg/kg L-DOC could inhibit the tumor volume better than that of same dose of DOC.In addition, 6mg/kg L-DOC and DOC didn’ t induce in vivo toxicity.Conclusion The L-DOC is prepared by film-ultrasonic dispersion method which has small diameter, great biocompatibility.And it could inhibit the HepG2 cells in vitro and in vivo, especially for no in vivo toxicity.
