Analysis the changes of SAA expression after liver transplantion by proteomics technique
- VernacularTitle:人肝移植急性排斥反应时 SAA 蛋白表达变化的蛋白质组学分析
- Author:
Yan LI
;
Yu LIU
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Acute rejection;
Serum;
Proteomics;
SAA
- From:
Journal of Chinese Physician
2015;(z1):4-6
- CountryChina
- Language:Chinese
-
Abstract:
Objective In this report the proteomics technique was used for analysis of the Molecu-lar marker related acute rejection after liver transplantation.Methods Highly abundant proteins in human serum were deleted using Aurum Serum Protein Mini Kit and separated by 2-DE.The maps were analyzed by ImageMaster 2D Platinum software.The differential proteins on the gel were did in gel digestion with trypsin and then identified by nanoUPLC-ESI-MS/MS.Results Reproducible 2-DE image was successfully ob-tained.2 up-regulated expressed protein spots were identified clearly are serum amyloid A protein.Conclu-sions In this study, it was confirmed that SAA expression upregulate after transplantation in patients with acute rejection by proteomics technique, This method may provide clues to the early diagnosis of liver al-lograft rejection.
