p53 inhibits the proliferation of lung cancer cell PC-9 by regulating miR-148b
10.3969/j.issn.1006-5725.2015.12.003
- VernacularTitle:p53通过调控miR-148b抑制肺癌细胞PC-9的增殖
- Author:
Yinjia FU
;
Xi YANG
;
Senyan LAI
;
Xiaonian CAO
;
Guihua WANG
;
Junbo HU
;
Xiang LI
- Publication Type:Journal Article
- Keywords:
Lung neoplasms;
p53;
miR-148b;
Cell proliferation
- From:
The Journal of Practical Medicine
2015;(12):1908-1911
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the function of p53 on regulating the expression of miR-148b in lung cancer cell line PC-9 and its corresponding molecular mechanism and the impact on cell proliferation. Methods Transient transfection of p53 eukaryotic expressing plasmids into lung cancer cell line PC-9 was performed to establish a cell model over-expressing p53. RT-PCR was used to explicit the impact of p53 on the expression of miR-148b. A reporter vector containing miR-148b promoter was used to investigate the function of p53 on regulating the transcription of miR-148b. Low-expressing miR-148b by transfecting its specific inhibitors , a CCK-8 assay was performed to explore the influence of miR-148b on the lung cancer cell proliferation inhibited by p53. Results Over-expression of p53 promoted miR-148b expression in lung cancer cell line PC-9. P53 could increase the luciferase activity driven by miR-148b promoters. Knockdown of miR-148b attenuated the impact of p53 on inhibiting the proliferation of PC-9 cells. Conclusion P53 inhibits the proliferation of lung cancer cell line PC-9 partially depending on miR-148b.
