Antiviral effects of pudilan xiaoyan oral liquid on respiratory syncytial virus and adenovirus in vitro
10.3969/j.issn.1006-5725.2015.11.037
- VernacularTitle:蒲地蓝消炎口服液对呼吸道合胞病毒和腺病毒的体外抗病毒作用
- Author:
Xuan WU
;
Li YU
;
Tao HU
;
Shenghai HUANG
- Publication Type:Journal Article
- Keywords:
Pudilan xiaoyan oral liquid;
respiratory syncytial virus;
adenoviruses serotype 3 strains;
anti-viral effect
- From:
The Journal of Practical Medicine
2015;(11):1838-1840
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the antiviral effects of Pudilan xiaoyan oral liquid on Hep-2 cell models infected with respiratory syncytial virus (RSV), adenoviruses serotype 3 strains (ADV3) in vitro. Methods The cell cytotoxic and inhibition effect of Pudilan xiaoyan oral liquid on RSV or ADV3 were investigated by MTT assay and the inhibitory assay for cytopathic effect (CPE) in Hep-2 cell cultures to detect its antiviral effects. Results The median toxic concentration (TC50) of Pudilan xiaoyan oral liquid on Hep-2 cells was 776.97 mg/L. The median effective concentration (EC50) of inhibiting RSV and ADV3 were 28.08, 28.10 mg/L,whose therapeutic index (TI) were 27.67 and 27.65 respectively. The safety coefficient of Pudilan xiaoyan oral liquid is higher than that of control, ribavirin. Compared with the virus control group, Pudilan xiaoyan oral liquid can obviously produce actions of a dose-dependent relationship to inhibit CPE in Hep-2 cells infected with RSV or ADV3 virus. Conclusions Pudilan xiaoyan oral liquid significantly improves the protection against RSV and ADV3 virus infection in Hep-2 cells. And the inhibition of CPE induced by viruses infection increased with the elevation of higher drug concentration for its antiviral effect augmented in vitro.
