The effect and significance of rhEPO to the expression of BCL-2 and Caspase-9 in the new type BPD model’ s lung tissue
- VernacularTitle:rhEPO对新型BPD模型肺组织BCL-2和Caspase-9表达的影响及意义
- Author:
Sifeng YUE
;
Hua ZHANG
- Publication Type:Journal Article
- Keywords:
bronchopulmonary dysplasia;
recombinant human erythropoietin;
apoptosis;
phosphatidylinositol 3-ki-nase/protein kinase B
- From:
Acta Universitatis Medicinalis Anhui
2015;(7):933-936
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT) pathway in recombinant human erythropoietin( rhEPO) protecting new type BPD. Methods Injected LPS or PBS to 40 spra-gue-dawley (SD) rats, gestational sacs on 15th day of gestation, the newborn rats were divided into four groups:control group, hyperoxia group, rhEPO group, rhEPO+LY294002 group. To detect the expression of lung tissue BCL-2 and Caspase - 9 protein and mRNA by Western blot and RT-PCR. Results In the hyperoxia group and rhEPO+LY294002 group, BCL-2 expression was decreased,while Caspase-9 expression was increased apparently;in the rhEPO group, BCL-2 expression was increased, while Caspase-9 expression was decreased. The differences between each group were statistically significant on 1st,7th and 14th day of hyperoxia exposure (P<0. 05). Con-clusion Intrauterine inflammatory exposure combined with hyperoxia after birth can cause lung cell apoptosis in-creases. While rhEPO has certain control effects on BPD, possibly by reducing the pulmonary apoptosis. The mechanism may be associated with PI3K/AKT signaling pathway.
