Application of DNA image cytometry in distinguishing benign and malignant pleuroperitoneal fluids
- VernacularTitle:细胞DNA定量分析在鉴别良恶性胸腹水中的应用价值
- Author:
Wei TAO
;
Jun LI
- Publication Type:Journal Article
- Keywords:
DNA image cytometry;
liquid-based cytology;
peritoneal effusion;
pleural effusion
- From:
Acta Universitatis Medicinalis Anhui
2015;(7):1016-1019
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the value of DNA image cytometry in thediagnose of benign and malignant pleuroperitoneal fluids by comparing with the liquid-based cytological results. Methods There were 417 cases in-volved in this study. Pap stain for cytology analysis and feulgen stain for DNA image cytometry were used to identify benign and malignant pleuroperitoneal fluids respectively,then compared the results of the two methods. Results Clinically or pathologically,213 were classified as malignant and the other 204 as benign. The sensitivity, specifici-ty,accuracy, positive predictive value and negative predictivevalue of DNA image cytometry were 89. 7%,100%, 94. 7%,100%,and 90. 3%,respectively. However,the sensitivity,specificity,accuracy, positive predictive value and negative predictive value of liquid-based cytology were 63. 4%, 81. 9%, 72. 4%, 78. 5%, and 68. 2%, re-spectively. There were significant differences in thesensitivity, specificity,accuracy, positive predictive value and negative predictivevalue. Conclusion DNA image cytometry has great application value in the diagnosis of benign and malignant pleuroperitoneal fluids, and can increase the positive rate, reduce misdiagnosed rate with liquid-based cytology.
