Study of Microelectrode Array Probe for Simultaneous Detection of Glutamate and Local Field Potential during Brain Death
10.11895/j.issn.0253-3820.150039
- VernacularTitle:脑死亡过程中谷氨酸与场电位的同步检测微电极阵列研究
- Author:
Wenjing WEI
;
Yilin SONG
;
Xinyi FAN
;
Song ZHANG
;
Li WANG
;
Shengwei XU
;
Xinxia CAI
- Publication Type:Journal Article
- Keywords:
Implantable microelectrode array;
Micro-electro-mechanical-systems;
Brain death;
High potassium;
Glutamate;
Local field potential
- From:
Chinese Journal of Analytical Chemistry
2015;(7):983-988
- CountryChina
- Language:Chinese
-
Abstract:
High extracellular potassium can induce spreading depression-like depolarizations, elevations of extracellular glutamate and even neuronal death in normal brain. To investigate the contribution of high potassium in vivo, a microelectrode arrays ( MEAs ) probe integrated with recording sites for glutamate concentration (50í150 μm) and local field potential ( LFP) ( diameter=15 μm) was fabricated by Micro-electro-mechanical-systems ( MEMS) technologies. We implanted the MEA probe acutely in the rat brain and exposed the brain to a high potassium solution. During these multi-modal recordings, it was observed that high potassium elevated extracellular glutamate while suppressing the LFP irreversibly. This is one of the first studies in which a dual mode MEA probes is applied in vivo for neuronal death, and it is concluded that our MEA probes are capable of examining specific spatiotemporal relationships between electrical and chemical signaling in the brain.
