Evaluation on Immune Fluorescence Chromatography for Glycosylated Hemoglobin

VernacularTitle:免疫荧光层析法测定糖化血红蛋白的效果评价
Author: Donglin CAO ; Da LEI ; Zhihong ZHANG ; Ting WANG ; Liang SUN
Publication Type:Journal Article
Keywords: Glycosylated hemoglobin; Immune fluorescence chromatography; High performance liquid chromatography
From: The Journal of Practical Medicine 2015;(11):1849-1851
CountryChina
Language:Chinese
Abstract: Objective To evaluate the effect of immune fluorescence chromatography on glycosylated hemoglobin (HbA1c). Methods The precision of immune fluorescence chromatography was evaluated with samples of 6.0% and 8.0% fixed value. Group of High performance liquid chromatography (HPLC) as control, HbA1c for 200 samples of EDTA-K2 anti-coagulated whole blood were detected by immune fluorescence chromatography to synchronous blinded trial. Results As to the precise of immune fluorescence chromatography in the samples of 6% and 8%, values of coefficient of variation were 5.1% and 5.3%, respectively. The linear regression equation of immune fluorescence chromatography and HPLC was Y=-0.110+1.021X and the correlation coefficient was 0.982. 6.0% and 8.0% as the cut-off value, kappa values were 0.950 (P < 0.001) and 0.922 (P < 0.001), respectively. Conclusion Immune fluorescence chromatography and HPLC is consistent with detection of HbA1c, which can be used for clinical detection of HbA1c.