Determination of rosmarinic acid in common selfheal fruit-spike effective components of colon target tablets
10.3760/cma.j.issn.1673-4246.2015.07.014
- VernacularTitle:夏枯草有效组分结肠靶向片剂中迷迭香酸含量HPLC法测定
- Author:
Jiongliang CHEN
;
Li LIU
;
Desheng XU
- Publication Type:Journal Article
- Keywords:
Common selfheal fruit-spike;
pH-delay;
Colon-targeting;
Rosmarinic acid;
Quantitative determination
- From:
International Journal of Traditional Chinese Medicine
2015;(7):632-635
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish HPLC method to determine the content and the recovery of rosmarinic acid in common selfheal fruit-spike effective component of colon target tablets.Methods HPLC column was Diamonsil C18,200 mm×4.6 mm,5μm, wavelength was 330 nm, mobile phase methanol was 0.1% formic acid aqueous solution (45:55), flow rate was 1.0 ml/min, and the sample size was 20μl.Results The fine linear relation was shown at the rosmarinic acid methanol concentration of 2.99-119.6μg/ml, with 72 h in stable state. The method has fine precision, the spotting recovery was 95%-105%, andRSD was less than 2%. Conclusion The method has high specificity, meeting the demands for content determination.
