A Microfluidic Chip with Titanium Dioxide Membrane Enhanced Pseudo Valve Used for the Two-Dimensional Electrophoresis
10.11895/j.issn.0253-3820.150007
- VernacularTitle:含有二氧化钛薄膜增强虚拟阀的微流控芯片用于二维凝胶电泳分离
- Author:
Jianjia SU
;
Hong CHEN
- Publication Type:Journal Article
- Keywords:
Microfluidic chip;
Two-dimensional electrophoresis;
Interface;
Titanium dioxide
- From:
Chinese Journal of Analytical Chemistry
2015;(7):989-993
- CountryChina
- Language:Chinese
-
Abstract:
Two-dimensional electrophoresis performed on a microfluidic chip could reduce the consumption of sample and reagents, realizing high-throughput analysis in a very short time. During the isoelectric focusing ( IEF) separation, the buffer for the two different separation methods should be kept from being mixed. After the IEF, the protein was transferred from the IEF channel into the second dimension channels. Based on the pseudo valve structure, this paper reported a method to deposit a titanium dioxide membrane at the interface of two dimensional channels, which enhanced the performance of the pseudo valve. The IEF electrophoresis and SDS-polyacrylamide gel electrophoresis ( SDS-PAGE ) procedures were characterized respectively. The total separation took 10-15 min, and the protein's migration rate was linear with the migration time, and inversely proportional to the logarithm of protein molecular weight. Based on the above results, the differential two-dimensional electrophoresis was performed to test the deviation between different measurements.
