Analysis on bacterial culture of bronchoalveolar lavage fluid from 1 693 children with refractory pneumonia
10.3969/j.issn.1671-9638.2015.06.005
- VernacularTitle:1693例难治性肺炎患儿支气管肺泡灌洗液病原体培养分析
- Author:
Baoxing HUANG
;
Jikui DENG
;
Hongmei WANG
;
Yan ZHANG
;
Ruizhen ZHAO
;
Hongyu CHEN
;
Heping WANG
;
Dongli MA
- Publication Type:Journal Article
- Keywords:
bronchoalveolar lavage fluid;
bronchoscope;
refractory pneumonia;
drug resistance,microbial;
aetiology
- From:
Chinese Journal of Infection Control
2015;(6):379-382
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate pathogens and antimicrobial resistance of pathogens causing refractory pneumonia in children.Methods Children with refractory pneumonia who admitted to a hospital between May 2008 and December 2014 were performed bronchoscopy,and bronchoalveolar lavage fluid (BALF)were performed bacterial culture and antimicrobial resistance testing.Results 1 693 patients were recruited in the study,273 bacterial isolates were isolated from BALF speci-mens of 226 children,gram-positive bacteria accounted for 38.10% (104/273 ),the main gram-positive bacteria were Streptococcus pneumoniae (n=71)and Staphylococcus aureus (n=23);gram-negative bacteria accounted for 58.24%(159/273),including 44 isolates of Haemophilus parainfluenzae ,28 Klebsiella pneumoniae ,19 Escherichia coli ,and 17 Pseud-omonas aeruginosa ;10 isolates of fungi were also detected,8 of which were Candida albicans .The sensitivity of Streptococ-cus pneumoniae to quinolones,ceftriaxone and cefotaxime were high.Methicillin-resistant Staphylococcus aureus (MRSA) positive rate was 26.32%.ESBLs-producing rate of Haemophilus parainfluenzae and Klebsiella pneumoniae was 32.72% and 62.96% respectively.Conclusion The major pathogens causing refractory pneumonia were Streptococcus pneumoniae and Haemophilus parainfluenzae ,empirical treatment should be conducted accordingly,antimicrobial resist-ance should be considered if therapeutic effect is poor,and targeted therapy should be performed according to cultured re-sults and antimicrobial susceptibility testing result.