Research on the effects of piperine on proliferation and invasion of human bladder cancer T24 ;Cells
10.3969/j.issn.1005-6483.2015.06.026
- VernacularTitle:胡椒碱对人膀胱癌 T24细胞增殖及侵袭作用的研究
- Author:
Junjie ZHOU
- Publication Type:Journal Article
- Keywords:
bladder cancer;
piperine;
proliferation;
invasion;
apoptosis
- From:
Journal of Clinical Surgery
2015;(6):467-469
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of differentpiperine concentrationson the prolif-eration and invasion of bladder cancer T24 cells.Methods Bladder cancer T24 cells were treated with different concentrations of piperine(5,1 0,20,40,80,and 1 60μmol/L).Then MTT assay,Transwell exper-iments,and western blot were used to detect the expression of bax and bcl-2,and the proliferation and ap-optosis of T24 cells.Results After a 24-hour treatment of piperine,IC50 value was 38.73μmol/L.With the increaseof piperine concentrations,the activity of T24 cell was significantly inhibited in a dose-depend-ent manner.Westernblot showed that the expression of anti-apoptotic protein bcl-2 reduced,and the pro-apoptotic protein bax increased.Conclusion Piperine could inhibit the proliferation of bladder cancer T24 cells and promote the apoptosis of T24 cells.
