Physical-chemical properties and enhancement effect of Integrin αvβ3/CCR2 dual targeted ultrasound microbubbles:a vitro study
10.3760/cma.j.issn.1004-4477.2015.05.021
- VernacularTitle:Integrinαvβ3/CCR2双靶向超声微泡的制备、理化性质及体外寻靶实验
- Author:
Yingying LIU
;
Jinfeng XU
;
Fei YAN
;
Hui LUO
;
Xinxin ZENG
;
Zhanghong WEI
;
Huiyu LIU
- Publication Type:Journal Article
- Keywords:
Microbubbles;
Moleculat targeted therapy;
Integrinαvβ3;
iRGD;
Dual-targeted
- From:
Chinese Journal of Ultrasonography
2015;(5):440-444
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare Integrin αvβ3/CCR2 dual targeted microbubble,test physical-chemical properties,enhancement effect and targeting ability in vitro.Methods The dual targeted microbubbles (MBdual-target )with FITC labled iRGD and PE labled CCR2 were prapared,and non-target microbubbles as control (MBcontrol ) were prapered.Physical and chemical properties of two groups of microbubbles were tested,connectivity of peptides/antibodies and microbubbles were detected by fluorescence microscope and flow cytometry instrument.Enhancement effect and the stability of two groups of microbubbles was observed and compared in vitro.The affinity of MBdual-target and MBcontrol for bEnd.3 cells was investigated with light and fluorescent microscope.Results ①The particle size of MBdual-target was (0.93±0.23)μm,with no statistically significant difference compared with MBcontrol (P >0.05).②MBcontrol showed no fluorescent,while MBdual-target showed both clear green and red light,under fluorescent microscope.③There was no significant difference of gray scale of enhancement between MBdual-target and MBcontrol in vitro.④ It was showed that MBdual-target adhered to bEnd.3 cells in vitro experiment.Conclusions Integrinαvβ3/CCR2 dual targeted targeted microbubbles was successfully prepared and proved having good enhancement effect and targeting ability in vitro.
