Preparation of a murine model of systemic Candida albicans infection
10.3969/j.issn.1005-4847.2015.03.015
- VernacularTitle:系统性念珠菌感染小鼠模型的制备
- Author:
Yue WU
;
Hang LI
;
Meiling CHEN
;
Baoyong GONG
;
Yu ZHANG
;
Ren HUANG
- Publication Type:Journal Article
- Keywords:
Candida albicans;
Animal model;
Mouse;
Fungal infection
- From:
Acta Laboratorium Animalis Scientia Sinica
2015;(3):301-305
- CountryChina
- Language:Chinese
-
Abstract:
To establish a stable mouse model of systemic Candida infection and to set up related standard operation procedure .Methods ICR mice were infected with C.albicans or C.parapsilosis by tail vein injection after immunosuppres-sion by cyclophosphamide .The quality control key points included immunosuppression , strain preparation, inoculation doses and the route of inoculation .Survival analysis , bacterial loads and pathological examination were performed to evaluate the prepared model .Results The developed model showed fugal-specific lesions in multiple organs , especially in the kidneys revealed by histopathological examination .Conclusions A stable mouse model of systemic Candida albicans infection can be successfully established by following standardized operation procedure .This mouse model may provide a useful tool for studies on pathogenesis and immune defense of fungal infection and new anti-fungal drug development and so on .
