Effects of Osthole on Differentiation of Neural Stem Cells in vitro

VernacularTitle:蛇床子素对神经干细胞体外分化的影响
Author: Shaoheng LI ; Yu HU ; Yingjia YAO ; Yanan JIAO ; Liang KONG ; Qingping YANG ; Zhenyu TAO ; Jingxian YANG
Publication Type:Journal Article
Keywords: Osthole; Neural stem cells; Differentiation; Notch signal pathway
From: Herald of Medicine 2015;(7):856-860
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of osthole on neural stem cells ( NSCs) differentiation and explore the potential mechanism. Methods Brain-derived NSCs from newborn mice were isolated and cultured in vitro and determined by immunofluorescence. The P5 generations of NSCs were placed in culture solution with osthole at concentrations of (0,10,50, 100 μmol·L-1 ) . The neuron, astrocyte and oligodendroglia cell differentiation were determined by immunofluorescence. The mRNA expression of Notch 1 and its target genes Mash 1 and Neurogenin 2 were assessed by RT-PCR. Results The neurosphere displayed Nestin and Sox 2-postive by immunofluorescence, suggesting that the cultured cells were NSCs. Osthole promoted NSCs differentiating into more neuron(P<0. 01) and oligodendrocyte(P<0. 05), but not astrocyte. Meanwhile, osthole significantly reduced the mRNA expression of Notch 1(P<0. 01) and increased Ngn 2(P<0. 01)at the dose of 100 μmol·L-1. Conclusion Osthole enhances NSCs differentiating into more neuron and oligodendrocyte via probablly inhibiting Notch signal pathway.