Efficient preparation of a TXNIP knockout mouse model by transcription activator-like effector nucleases (TALEN)
- VernacularTitle:利用 TALEN 技术高效制备 TXNIP 基因敲除小鼠模型
- Author:
Huanhuan ZHANG
;
Chuxin LIU
;
Yue MA
;
Liping XIAO
;
Feida LI
;
Huazhong YING
;
Huan LIU
- Publication Type:Journal Article
- Keywords:
TALEN;
Microinjection;
TXNIP;
Gene knockout;
Model,mouse
- From:
Chinese Journal of Comparative Medicine
2015;(6):9-13
- CountryChina
- Language:Chinese
-
Abstract:
Objective To knockout the murine Txnip gene using microinjection of transcription activator-like effector nuclease ( TALEN) mRNAs.Methods TALEN knockout site recognizing Txnip was designed by tools on line, then constructed the vectors and assayed its cleavage activity at cellular level.TALEN mRNA was transcribed in vitro and microinjected into C57BL/6J mouse zygotes.F0 mice were verified at DNA level with BamHI and TXNIP-knockout mice were obtained.Results We designed and constructed TALENs which recognized and cut the first exon of Txnip, and got four TXNIP knockout mice, among which two were frameshift mutation, demonstrating that the TXNIP-knockout mice were generated by TALEN technique.Conclusions Microinjection of in vitro transcribed TALEN mRNAs into murine zygotes is a highly effective and convenient way to develop TXNIP-knockout mouse model.
