Effects of hyperoxia solution on pulmonary surfactant and histological changes in rabbits with oleic acid-induced acute lung injury
10.7652/jdyxb201504014
- VernacularTitle:高氧液对油酸性急性肺损伤家兔肺组织改变和肺表面活性物质的影响
- Author:
Yali CHEN
;
Dandan ZHOU
;
Hongxia CHENG
;
Yichang ZHANG
- Publication Type:Journal Article
- Keywords:
hyperoxia solution;
oleic-induced acute lung injury;
pulmonary surfactant;
surfactant protein A
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(4):496-500
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of hyperoxia solution on pulmonary surfactant and histology of rabbits with oleic acid-induced acute lung injury.Methods Thirty healthy rabbits weighing 2.0 - 2.5 kg were randomly divided into three groups with 10 rabbits each:control group,hyperoxia solution treatment group,and saline treatment group.Blood samples were taken for blood-gas analysis before and at 30,60 and 120 min after oleic acid or normal saline administration.Two hours later,the animals were killed and pathologic changes of lung tissue were observed microscopically.The expression of SP-A was investigated using the immunohistochemical method and image analysis system.Results PaO2 was significantly higher in hyperoxia solution treatment group than in saline treatment group two hours after treatment (P < 0.001 ),but PaCO2 was significantly lower than that in saline treatment group (P <0.001).Optical microscopy showed that lung tissue damage was milder in hyperoxia solution treatment group than in saline treatment group.The content of surfactant A in hyperoxia solution treatment group was significantly greater than that in saline treatment group (P < 0.001 ).Conclusion Hyperoxia solution can lessen lung tissue injury and damage to pulmonary surfactant.
