Effect of Hedyotic diffusa injection in reversing multi-drug resistance of human hepatoma BEL-7402/5-FU cells
10.7652/jdyxb201504026
- VernacularTitle:白花蛇舌草注射液逆转人肝癌细胞BEL-7402/5-FU 的多药耐药作用
- Author:
Zuozhuang LIAO
;
Limei WEI
;
Lingyuan XU
;
Gang LIANG
- Publication Type:Journal Article
- Keywords:
Hedyotic diffusa;
hepatoma cell;
multi-drug resistance;
apaptosis;
tyrosine kinases
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(4):554-557
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect and mechanism of Hedyotic diffusa injection in reversing multi-drug resistance of BEL-7402/5-FU cells.Methods The IC50 of BEL-7402/5-FU cells was examined by MTT,apoptosis was detected by flow cytometry,and the content of tyrosine kinase in total protein of tumor cells was detected by enzyme-linked immunosorbent assay (ELISA).Results The IC50 of Hedyotic diffusa injection (6 μL/mL ) combined with 5-FU (636.5 μmol/L)decreased significantly compared with that of 5-FU applied alone (1 071.6 6μmol/L). The reversal on BEL-7402/5-FU was 1.68-fold.After injection of 5-FU plus Hedyotic diffusa ,the apoptosis rate of BEL-7402/5-FU cells was increased significantly and the tyrosine kinases of BEL-7402/5-FU cells decreased significantly.Conclusion Hedyotic diffusa injection plays a certain role in reversing multi-drug resistance of BEL-7402/5-FU cells,which may be related to decreasing their tyrosine kinases.
