Construction and identification of Mcl-1 gene shRNA expression plasmid in targeted silenced macrophages
10.7652/jdyxb201504027
- VernacularTitle:靶向沉默巨噬细胞中 Mcl-1基因 shRNA表达质粒的构建与鉴定
- Author:
Chan WANG
;
Xinmin WANG
;
Feiyu WANG
;
Yuqing ZHANG
;
Xudong CAO
;
Jiangdong WU
;
Fang WU
;
Wanjiang ZHANG
;
Le ZHANG
- Publication Type:Journal Article
- Keywords:
myeloid cell leukemia-1;
Raw264.7 cell;
THP-1 cell;
Mycobacterium Tuberculosis;
short hairpin RNA
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(4):558-564
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expressions of myeloid cell leukemia-1 (Mcl-1 ) gene in mouse macrophages Raw264.7 and human macrophages THP-1,to screen out the cell lines with high levels of expression as the experimental cells,and based on the screening results to construct the short hairpin RNA(shRNA)eukaryotic expression plasmid targeting mice Mcl-1 gene for transfection and further screen out the shRNA expression plasmid with the most obvious effect in silencing Mcl-1 gene.Methods Semi-quantitative PCR method was used to detect the expression of Mcl-1 mRNA in the two kinds of macrophages.Western blotting was adopted to detect the expressions of Mcl-1 proteins in the two kinds of macrophages.Three different gene loci for Mcl-1 shRNA fragments were designed with small molecules interfering RNA (siRNA)software.Eukaryotic expression plasmid Mcl-1 shRNA 1-3 carrying the shRNA fragments was constructed by a company.And the eukaryotic expression plasmid vector was transfected into scavenger cells Raw264.7 mice via through the liposome.The transfection results 24 h and 48 h after transfection were observed under the inverted fluorescence microscope;Mcl-1 mRNA and protein expression were detected by real time quantitative PCR and Western blot,respectively.Results The relative expression levels of Mcl-1 mRNA and protein in mouse macrophages Raw264.7 were significantly higher than those of human macrophages (P <0.05).The shRNA expression vector constructed within 24 h and 48 h could decrease Mcl-1 mRNA and protein levels in Raw264.7 cells,especially with the most obvious silencing effect at 48 h.The 48-h transfection group differed significantly from normal group,liposome group and negative control group (P <0.05).Compared with Mcl-1shRNA1and Mcl-1shRNA2,Mcl-1shRNA3 had the strongest effect in silencing Mcl-1mRNA and protein.Conclusion We have successfully screened out experimental Raw264.7 cells and Mcl-1 shRNA eukaryotic expression plasmid which has an obvious silencing effect targeting on Mcl-1 in mice macrophages Raw264.7.
