Determination of Diosbulbin B in Dioscorea bulbifera L. from Different Regions
10.3870/yydb.2015.07.025
- VernacularTitle:不同产地黄药子中黄独乙素的含量测定
- Author:
Xiongrong SHEN
;
Jianyong DONG
- Publication Type:Journal Article
- Keywords:
Dioscorea bulbifera L.;
Diosbulbin B;
Content determination;
Chromatography,high performance liquid
- From:
Herald of Medicine
2015;(7):934-937
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for determining and comparing the contents of diosbulbin B in
Dioscorea bulbifera L. from different regions. Methods The medicine powders were extracted twice by water, then the water extracts were combined and detected by HPLC at 35℃,with Kromasil-C18 column (4. 6 mm×250 mm,5μm) and a mobile phase of acetonitrile-water-glacial-acetic acid (34660. 1). The flow rate was 1. 0 mL·min-1 and the detection wavelength was 210 nm. Results The linear range of diosbulbin B was 26. 0-260. 0 μg·mL-1(r=0. 999 9). The average content of diosbulbin B in Dioscorea bulbifera L. from different areas was that of Hebei>Guangxi>Jiangsu>Sichuan>Zhejiang. Conclusion The method is simple,quick,accurate and suitable for the determination of diosbulbin B in Dioscorea bulbifera L. from different regions.
