Bone tissue regeneration of bone morphogenetic protein-7-transfected bone marrow mesenchymal stem cells
10.3969/j.issn.2095-4344.2015.23.001
- VernacularTitle:骨形态发生蛋白7转染骨髓间充质干细胞再生骨组织
- Author:
Ping WANG
;
Yucen GU
;
Zhihui GAO
;
Tiefeng SUN
;
Wubin YANG
- Publication Type:Journal Article
- Keywords:
Bone Marrow;
Mesenchymal Stem Cells;
Bone Morphogenetic Protein 7;
Lentivirus Infections
- From:
Chinese Journal of Tissue Engineering Research
2015;(23):3609-3615
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Bone morphogenetic protein-7 can promote proliferation of bone marrow mesenchymal stem cels and has important significance in terms of inducing new bone formation. OBJECTIVE:To observe the protein and mRNA expression of bone morphogenetic protein-7 in bone marrow mesenchymal stem cels transfected with bone morphogenetic protein-7. METHODS:Rat bone marrow mesenchymal stem cels were isolated and cultured, and then identified by the morphology and cel surface markers. Lentiviral vectors carrying bone morphogenetic protein-7 were constructed to transfect bone marrow mesenchymal stem cels. After total RNA extraction, the protein and mRNA expression of bone morphogenetic protein-7 was detected by RT-PCR and western blot methods. RESULTS AND CONCLUSION:PLV-sfGFP(2A)-BMP7 recombinant plasmids were successfuly constructed and transferred into rat bone marrow mesenchymal stem cels. The mRNA and protein expression of bone morphogenetic protein-7 in the transfection group was significantly higher than that in the blank vector and blank groups (P < 0.05), indicating exogenous bone morphogenetic protein-7 gene is effectively integrated into the bone marrow mesenchymal stem cels.
