Differentiation of bone marrow and umbilical cord blood mesenchymal stem cells into osteoblasts in vitro：comparison of their osteogenic potentials
10.3969/j.issn.2095-4344.2015.23.008
- VernacularTitle:体外诱导骨髓与脐血间充质干细胞向成骨细胞分化及其成骨活性的比较
- Author:
Shuai SHAO
;
Chenhong ZHOU
;
Lili XU
- Publication Type:Journal Article
- Keywords:
Mesenchymal Stem Cells;
Fetal Blood;
Bone Marrow;
Osteoblasts;
Cell Differentiation
- From:
Chinese Journal of Tissue Engineering Research
2015;(23):3652-3657
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Mesenchymal stem cels isolated from cord blood and bone marrow have multi-directional differentiation ability under a certain condition of induction. OBJECTIVE:To compare the difference of differentiation of umbilical cord blood and bone marrow mesenchymal stem cels into osteoblasts. METHODS:Human umbilical cord blood and bone marrow mesenchymal stem cels were isolated and cultured by density gradient method. When reached 90% confluency, mesenchymal stem cels were digested by trypsin for subculture. At the third passage, umbilical cord blood mesenchymal stem cels and bone marrow mesenchymal stem cels at 8×104/wel were incubated. When reached 80% confluency, cels were treated with low-glucose DMEM supplemented with 10% fetal bovine serum, 0.1 μmol/L dexamethasone, 50 μmol/L vitamin C and 10 mmol/L β-sodium glycerophosphate. RESULTS AND CONCLUSION:There was no significant difference in morphology and biological properties of the two kinds of mesenchymal stem cels. Cels were highly expressed CD44, CD29, but did not express CD34. They had the ability to differentiate into osteoblasts, which had a positive staining for known markers: alkaline phospatase and calciumin vitro mineralization. There was no significant difference in the activity of osteoblasts of two kinds of cels. Results verify that umbilical cord blood and adult bone marrow mesenchymal stem cels can be induced into osteoblasts with a similar ability, and they can be used as seed cels for bone tissue engineering.
