Biocompatibility of porcine bone marrow mesenchymal stem cells-derived bile duct endothelial cells with electrospun nanofibers
10.3969/j.issn.2095-4344.2015.23.022
- VernacularTitle:骨髓间充质干细胞诱导胆管内皮细胞与电纺纳米纤维的生物相容性
- Author:
Yang YANG
;
Jiahua ZHOU
;
Xueyan YIN
;
Yong XU
;
Yang CAO
;
Qian XU
- Publication Type:Journal Article
- Keywords:
Bone Marrow;
Mesenchymal Stem Cells;
Cell Differentiation;
Bile Duct Endothelial Cells;
Nanofibers;
Biocompatible Materials
- From:
Chinese Journal of Tissue Engineering Research
2015;(23):3736-3743
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Repair of extrahepatic biliary tract injury is a difficult problem in the abdominal surgery. Tissue-engineered extrahepatic biliary tract is an ideal selection for this problem. Construction of tissue-engineered extrahepatic biliary tract with excelent performance is a key to related studies. OBJECTIVE:To investigate the biocompatibility of bile duct endothelial cels differentiated by porcine bone marrow mesenchymal stem cels with electrospun nanofibers. METHODS:Porcine bone marrow mesenchymal stem cels were induced toward biliary tract endothelial cels, which were then identified by morphology and RT-PCR. Polylactic-co-glycolic acid (PLGA) nanofiber membranes were prepared by electrospinning. The morphology was determined by scanning electron microscopy and the short-term (2-week)in vitro degradation rate was determined. Adhesion and proliferation of biliary tract endothelial cels on the nanofiber surface was analyzed by calculating the cel adhesion rate and MTT assay, respectively. Cel growth, morphology and distribution on the material surface were observed by fluorescence staining and scanning electron microscopy, respectively. RESULTS AND CONCLUSION: After 4 weeks of directed differentiation of bone marrow mesenchymal stem celsin vitro, cels showed typical morphology of dendritic bile duct endothelial cels and had the expression of CK19. Scanning electron micrographs showed that electrospun materials were continuous nanofibers with diameters between 200 and 500 nm. No significant degradation of the PLGA nanofibers was observed within 2 weeks. Based on the measured cel adhesion rate, MTT assay, fluorescence staining, and scanning electron microscopy, the differentiated cels possessed a good proliferative capacity on PLGA nanofibers. Bone marrow mesenchymal stem cels differentiated into bile duct endothelial cels in vitro. Materials prepared by the electrospinning method had a nanofiber structure, which did not significantly degrade within 2 weeks. Differentiated cels exhibit good biocompatibility with the nanofibers.
