Research of Ginsenoside on proliferation inhibitory of cultured tumor stem cells of human rectal carcinoma in vitro
10.3760/cma.j.issn.1008-6706.2015.15.002
- VernacularTitle:人参皂苷对体外培养直肠癌肿瘤干细胞增殖抑制的研究
- Author:
Ruoming WANG
;
Zhen YANG
- Publication Type:Journal Article
- Keywords:
Gisenosides;
Rectal neoplasms;
Tumor stem cells
- From:
Chinese Journal of Primary Medicine and Pharmacy
2015;(15):2244-2246
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe inhibitory effects of Ginsenoside on the proliferation cultured stem cells of human rectal carcinoma in vitro.Methods Tumor stem cells of rectal carcinoma in human were cultured in vitro and divided into saline water group,reltitrexed group(3mg/mL),and Ginsenoside(25μg/mL)plus reltitrexed(3mg/mL) group,with dosage of qd and for 2 weeks.Cells growth was observed under the microscope,and immunofluorescence staining was used to detect CD +133 expression of rectal carcinoma cells.MTT colorimetric method was taken to measure inhibitory of Ginsenoside on proliferation of tumor stem cells of rectal carcinoma.Apoptosis of tumor stem cells of rec-tal carcinoma lead by Ginsenoside was observed by DAPI staining.Results Part of cells were growing up like ball and CD +133 immunofluorescence staining was positive.Inhibitory role of Ginsenoside plus reltitrexed on CD +133 cells of rectal carcinoma appeared on the first day,which was bigger than that of retitrexed by MTT method (P =0.03).Com-pared with the retitrexed group,Gisenoside combined with reltitrexed reduced CD +133 cells expression of rectal carcino-ma,in which optical density and area density of CD +133 cells were obviously decreased with statistical significance (P =0.007,P =0.006,respectively).Conclusion Gisenoside plays the remarkably inhibitory role on proliferation of CD +133 cells of rectal carcinoma.
