Effects of mycophenolate mofetil on anti-glomerular basement membrane nephritis SD rats
10.3969/j.issn.1671-8348.2015.18.007
- VernacularTitle:吗替麦考酚酯对SD大鼠抗肾小球基底膜肾炎模型的治疗效果
- Author:
Xun FANG
;
Changxuan LIU
;
Wenli CHEN
- Publication Type:Journal Article
- Keywords:
mycophenolate mofetil;
anti-glomerular basement membrane disease;
clomerulonephrits;
renal insufficiency;
in-terleukin-1
- From:
Chongqing Medicine
2015;(18):2467-2470
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of mycophenolate mofetil(MMF) on blood and urine biochemical indica‐tors as well as the expressions of IL‐1 in anti‐glomerular basement membrane(GBM ) nephritis SD rats .Methods The SD rats were randomly divided into control group ,model group and MMF group .And then the models of SD rat anti‐GBM nephritis were estab‐lished by injecting rabbit anti‐SD rat GBM serum antibodies .The urinary protein and blood parameters were detected initially ,1st , 2nd ,3rd and 4th weeks after administration respectively .The expressions of IL‐1 and TGF‐β1 were detected by Elisa Kit and the re‐nal pathological changes were observed by HE and PAS staining .Results After treatment for 4 weeks by MMF ,the 24 h UP ,BUN and SCr levels were lower in MMF group than those of the model group(P<0 .05) .And the expressions of IL‐1 and TGF‐β1 were decreased in MMF group .The results of HE and PAS staining showed that in model group rats ,glomerular tuft morphology was destroyed and inflammatory cell infiltration was observed in the glomeruli .Some glomeruli had cellular crescent formation .Infiltra‐tion of inflammatory cells was also observed in the interstitium .MMF clearly improved these pathological changes overall ,with im‐provements noted in both glomerular and tubular degeneration as well as in the infiltration of inflammatory cells .Conclusion MMF shows certain renal protective effect on anti‐GBM nephritis SD rats .
