The protection study of recombinant human erythropoietin on acute cerebral injury rats

VernacularTitle:重组人促红细胞生成素对急性脑损伤大鼠的神经保护作用研究
Author: Daihua YU ; Xude SUN ; Wei CAI ; Chanjun GAO
Publication Type:Journal Article
Keywords: erythropoietin; brain injury; glutamate transporters 1; aspartate aminotransferases
From: Chongqing Medicine 2015;(18):2474-2476
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of recombinant human erythropoietin(rh‐EPO ) on acute cerebral injury and expression of GLT‐1 and GLAST in rat .Methods Sixty SD rats were randomly divided into three groups by weight :control group (n=18) ,acute cerebral injury group(n=22) and rh‐EPO conditioning group(n=20) .Acute cerebral injury models were made by modified Feeney′s method .rh‐EPO was injected in abdominal cavity 15 min after acute cerebral injury in rh‐EPO conditioning group .Rats′brain were removed 48 h after experiments .Rat GLT‐1 and GLAST mRNA expression were determined by RT‐PCR , GLT‐1 and GLAST protein expression were determined by Western blot .Results GLT‐1/GLAST mRNA and protein expression decreased significantly after acute cerebral injury(all P<0 .01) ,but increased significantly in rh‐EPO preconditioning group com‐pared with acute cerebral injury group(all P<0 .01) .Conclusion rh‐EPO preconditioning may protect against acute cerebral injury by up regulating the expression of GLT‐1/GLAST .