The related research of interferon -inducible genes MNDA in patients with systemic lupus erythematosus
10.3760/cma.j.issn.1008-6706.2015.13.009
- VernacularTitle:干扰素诱导基因 MNDA 表达与系统性红斑狼疮的相关性研究
- Author:
Guiqi ZHU
;
Meixia LIU
;
Nali HOU
;
Yinhuan ZHAO
;
Ling WANG
- Publication Type:Journal Article
- Keywords:
Systemic lupus erythematosus;
Interferon -inducible genes;
reverse transcription polymerase chain reaction
- From:
Chinese Journal of Primary Medicine and Pharmacy
2015;(13):1944-1946
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression levels of interferon -inducible genes (MNDA)in the peripheral blood mononuclear cells (PBMCs)of patients with systemic lupus erythematosus (SLE),and the relations between the gene expression levels and disease activity were explored.Methods Reverse transcription polymerase chain reaction(RT -PCR)method was used to detect the expression levels of MNDA in 30 patients with SLE and 25 controls.The associations between the expression levels of MNDA and urine protein,complement C3,C4,anti -double stranded DNA antibody and SLEDAI scores in patients with SLE were analyzed.Results (1)The expression level of MNDA mRNA in the SLE patients was significantly higher than that of the normal controls(t =6.99,P <0.01).(2)The expression level of MNDA in the urinary protein positive patients was significantly higher than that of the urinary protein negative patients (t =3.08,P <0.01),they were all significantly higher than the normal controls (t =9.32,4.87,all P <0.01).(3)The expression levels of MNDA mRNA were positively correlated with anti -double stranded DNA antibody and SLEDAI scores(r =0.534,0.508,all P <0.05),and not correlated with comple-ment C3 and C4(r =-0.472,-0.349,all P >0.05 ).Conclusion The expression levels of MNDA mRNA in patients with SLE are significantly higher than those of the normal controls.It is linked to kidney damage and disease activity,and helpful in evaluating SLE disease activity and severity.
