The expression and correlation of miR-335 and ROCK1 in osteosarcoma
10.3969/j.issn.1671-8348.2015.16.004
- VernacularTitle:miR-335及ROCK1在骨肉瘤中表达及相互关系研究
- Author:
Yong WANG
;
Kefeng WANG
;
Wei ZHAO
- Publication Type:Journal Article
- Keywords:
microRNA-335;
rho-associated kinases 1;
osteosarcoma;
gene expression
- From:
Chongqing Medicine
2015;(16):2170-2173
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and correlation of microRNA‐335(miR‐335) and ROCK1 in osteosarco‐ma tissue and osteosarcoma cell lines MG‐63 and U2‐OS .Methods Eighteen paired tissue specimens of osteosarcoma and matched normal tissue were chosen clinically .Two osteosarcoma cell lines MG‐63 and U2‐OS were chosen as experimental group while the human osteoblast cell line hFOB 1 .19 as control group ,Quantitative real‐time PCR were used to evaluate the different expression of miR‐335 and ROCK1 mRNA in osteosarcoma tissue and cell lines;miR‐335 mimic was transfected into MG‐63 and U2‐OS ,while Quantitative real‐time PCR and Western blot were used to evaluate the effect of over‐expressed miR‐335 on ROCK1 mRNA and protein level .Results The result of real‐time PCR indicated a special lower expression of miR‐335 but higher ROCK1 ,and the cor‐relation between miR‐335 and ROCK1 was negative .Western blot showed that miR‐335 could decrease ROCK1 protein expression . miR‐335 mRNA showed a hyper‐expression while lower ROCK1 mRNA and protein after transfection of miR‐335 mimic .Conclusion The expression of miR‐335 and ROCK1 shows a negative correlation in both tissue and cell level;over‐expression of miR‐335 can decrease ROCK1 expression .
