Liquid chromatography-tandem mass spectrometry method for the estimation of adefovir in human plasma：Application to a pharmacokinetic study
- Author:
Dipanjan GOSWAMI
;
Sanjay GURULE
;
Arabinda SAHA
;
Poonam VATS
;
Arshad KHUROO
;
Tausif MONIF
- Publication Type:Journal Article
- Keywords:
Adefovir;
Liquid chromatography-tandem mass spectrometry;
Solid phase extraction;
Pharmacokinetic study
- From:
Journal of Pharmaceutical Analysis
2015;(3):190-199
- CountryChina
- Language:Chinese
-
Abstract:
An analytical method based on solid phase extraction was developed and validated for analysis of adefovir in human plasma. Adefovir-d4 was used as an internal standard and Synergi MAX RP80A (150 mm × 4.6 mm, 4μm) column provided the desired chromatographic separation of compounds followed by detection with mass spectrometry. The method used simple isocratic chromato-graphic condition and mass spectrometric detection in the positive ionization mode. The calibration curves were linear over the range of 0.50–42.47 ng/mL with the lower limit of quantitation validated at 0.50 ng/mL. Matrix effect was assessed by post-column infusion experiment to monitor phospholipids and post-extraction addition experiment was performed. The degree of matrix effect for adefovir was determined as 7.5%and ion-enhancement in five different lots of human plasma was 7.1%and had no impact on study samples analysis with 4.5 min run time. The intra- and inter-day precision values were within 7.7% and 7.8%, respectively, for adefovir at the lower limit of quantification level. Validated bioanalytical method was successfully applied to clinical sample analysis.
