Effect of lavage with artificial cerebrospinal fluid on neural cell apoptosis and the ERK pathway after rat traumatic brain injury
10.11817/j.issn.1672-7347.2014.11.002
- VernacularTitle:鼠脑外伤后人工脑脊液灌洗对ERK通路和神经细胞凋亡的影响
- Author:
Jiaode JIANG
;
Yunsheng LIU
;
Wenhua FANG
- Publication Type:Journal Article
- Keywords:
artiifcial cerebrospinal lfuid;
neural cells apoptosis;
ERK
- From:
Journal of Central South University(Medical Sciences)
2014;(11):1111-1117
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of lavage with artiifcial cerebrospinal lfuid on neural cell apoptosis and the extracellular regulated kinase (ERK) pathway atfer traumatic brain injury. Methods: A total of 192 SD rats were randomly divided into a sham group, a traumatic brain injury model group, a local artiifcial cerebrospinal lfuid group, and a local saline group. Each group was divided into 4 sub-groups by the sacriifced time at 6 h, 12 h, 1 d and 3 d atfer the operation. hTe phosphorylation of extracellular regulated kinase 2 (P-ERK2), TNF-α and cellular apoptosis were examined. Results: hTe levels of P-ERK2 protein and TNF-α protein, as well as the number of apoptotic cellsat each time point in the local artiifcial cerebrospinal lfuid group were lower than those in the model group or in the saline group (P<0.05). Conclusion: Lavage with artificial cerebrospinal fluid can reduce apoptosis of neural cells after brain injury through the ERK pathway.
