Analysis of the usage of TCRβ V/J subfamily and CDR3 repertoire in patients with peripheral T-cell lymphoma unspecified

VernacularTitle:外周T细胞淋巴瘤非特指型TCRβ V/J取用及CDR3谱型分析
Author: Jianbo ZHANG ; Yongping SONG ; Qingkai YU ; Xiaodong LYU ; Jun HU
Publication Type:Journal Article
Keywords: T-cell lymphoma; Multi-PCR; Complementarity determining region
From: Journal of Leukemia & Lymphoma 2014;23(8):465-467
CountryChina
Language:Chinese
Abstract: Objective To analyze the restricted usage of TCRβ V/J subfamily and CDR3 repertoire in patients with peripheral T-cell lymphoma unspecified (U-PTL).Methods The total RNA was respectively extracted from lymph node of U-PTL and reverse transcriptase,then multi-PCR was used to amplify the complete DNA sequence(CDS) of TCR β-chain.The recombinant plasmids were sequenced and sequence was analyzed by using online TCR resources.Results There were 9 TCR β chain CDS obtained from four patients.TCRβ-chain presented specific repertoire skewing in patients with U-PTL.There were restricted usage of BV2,BV4S2,BV14,BV29S1 of BV subfamily and BJ1S4,BJ2S3,BJ2S5,BJ2S7 of BJ subfanily.The clonal proliferation T cells had different CDR3 amino acid sequences.Conclusions There are restricted usage of TCR β V/J subfamily in patients with U-PTL.The sequences of CDR3 in different TCR clone proliferation are mostly different.