Expression of pituitary tumor transforming gene and c-myc genes in non-Hodgkin lymphoma patients
10.3760/cma.j.issn.1009-9921.2014.06.008
- VernacularTitle:非霍奇金淋巴瘤患者骨髓垂体瘤转化基因、c-myc基因表达的研究
- Author:
Huali DONG
;
Nan LYU
;
Daqi LI
;
Ping CHEN
;
Jianhua SHAO
;
Xuebin DONG
- Publication Type:Journal Article
- Keywords:
Lymphoma,non-Hodgkin;
Pituitary tumor transforming gene;
Genes,myc;
Gene expression
- From:
Journal of Leukemia & Lymphoma
2014;23(6):350-353
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of pituitary tumor transforming gene (PTTG) and c-myc gene in patients with non-Hodgkin lymphoma (NHL),its relationship with NHL and its clinical significance.Methods PTTG mRNA and c-myc mRNA levels in bone marrow mononuclear cell (BMMNC) isolated from 38 NHL patients and 10 chronic lymphadenitis patients were quantified by reverse transcriptionpolymerase chain reaction (RT-PCR).Results mRNA expression of PTTG and c-myc gene in BMMNC was significantly higher in NHL patients than those in normal controls (PTTG:0.567 7±0.270 7 vs 0.071 2± 0.020 1,t =4.706,P ＜ 0.05; c-myc:0.352 6±0.185 4 vs 0.107 3±0.043 5,t =3.303,P =0.002).The expression of PTTG and c-myc gene in peripheral T cell lymphoma and diffuse large B cell lymphoma showed no significant difference (PTTG:0.556 8±0.211 3 vs 0.602 8±0.244 6,t =0.640,P =0.527; c-myc:0.350 1± 0.177 6 vs 0.361 0±0.190 2,t =0.302,P =0.765).Both expression of PTTG and c-myc were positively related to NHL clinical stage and IPI.Expression of PTTG mRNA was positively related to the expression of c-myc mRNA.Conclusion There was overexpression of PTTG and c-myc in NHL,which indicates that PTTG might be involved in tumorigenesis of NHL through direct or indirect activation of c-myc oncogene.
