Effect of hydroxychloroquine on the expression of suppressors of cytokine signaling in MRL/lpr mice kidney
10.3760/cma.j.issn.1007-7480.2014.11.013
- VernacularTitle:硫酸羟氯喹对MRL/lpr狼疮小鼠肾组织细胞因子信号抑制因子表达的影响
- Author:
Xin FENG
;
Wei GAO
- Publication Type:Journal Article
- Keywords:
Lupus nephritis;
Suppressors of cytokine signaling proteins;
Hydroxychloroquine
- From:
Chinese Journal of Rheumatology
2014;18(11):773-776
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of hydroxychloroquine (HCQ) on the suppressors of cytokine signaling (SOCS) in the kidney of MRL/lpr mice and evaluate the protection of HCQ on lupus nephritis.Methods Twenty-six rats were randomly divided into 3 groups:the control group which was consisted of 6 mice; the MRL/lpr group which was consisted of 10 mice; and the HCQ group which was consisted of 10.24-hour urinary protein level and anti dsDNA levels were tested.The expressions of SOCS-1 and SOCS-3 protein were determined by western blot.Analysis of variance was used to determine statistically significant differences between the two groups.A P value less than 0.05 was considered to be statistically significant.Results Compared with the control group,the levels of 24-hour urinary protein [(2 356.6±509.3) mg vs (440.3±90.1)mg] and anti ds-DNA (128.7±32.3 vs 14.9±1.4) were significantly increased in the MRL/lpr group,the expression levels of SOCS-1 and SOCS-3 protein were increased.After treated with HCQ,the levels of 24-hour urinary protein [(70.8±11.1) mg vs (2 356.6±509.3) mg] and anti-dsDNA (111.8±330.1 vs 128.7±32.3)were significantly decreased,the expression level of SOCS-1 and SOCS-3 protein decreased significantly (P＜ 0.05).Conclusion HCQ can inhibit SOCS expression in the kidney of lupus mice,reduce proteinuria and delay progression of immune disorders and lupus nephritis.
