Effects of Tanshinone ⅡA on proliferation and apoptosis of myeloblastic leukemia cell lines
10.3760/cma.j.issn.1009-9921.2014.06.004
- VernacularTitle:丹参酮ⅡA抑制髓系白血病细胞株增殖及诱导凋亡的研究
- Author:
Hui LI
;
Mei LIU
;
Hongjia LI
;
Wei LI
;
Wenjin XIE
- Publication Type:Journal Article
- Keywords:
Leukemia;
Tanshinone ⅡA;
Apoptosis;
Cell cycle;
Caspase 3
- From:
Journal of Leukemia & Lymphoma
2014;23(6):334-338
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of Tanshinone Ⅱ A (Tan Ⅱ) on proliferation and apoptosis of myeloblastic leukemia cell lines.Methods NB4,K562 and THP-1 cells were incubated with TanⅡA for 24,48 and 72 hours.Ddaunorubicin was used as a positive control.Cell proliferation was monitored by MTT assay.Cell apoptosis and cell cycle were determined by Annexin Ⅴ-FITC/PI flow cytometry.Expression of Caspase-3 was quantified by spectrophotometry.Results After incubation with various leukemia cells for 24,48 and 72 hours,Tan Ⅱ inhibited proliferation of NB4 cells with IC50 of 24.11,9.60 and 7.28 μmol/L,inhibited K562 cells with IC50 of 31.75,11.88 and 6.81 μmol/L and inhibited THP-1 cells with IC50 of 111.10,32.82 and 11.82,respectively.After treatment with Tan Ⅱ for 48 hours,cell apoptosis,the number of G1 phase cells and the expression of Caspase-3 in all three leukemia cell lines were increased significantly comparing with the blank control group (P ＜ 0.05).Conclusions Tan Ⅱ A has proliferation inhibitory effect on myeloblastic leukemia cell lines by the order of effect NB4＞K562＞THP-1.Tan ⅡA displays anti-leukemia activity possibly through arresting leukemia cells in G1 phase and inducing apoptosis by increasing Caspase-3 expression.
