Levels of interleukin-8, interleukin-4 and tumor necrosis factor-α of neonatal mouse with systemic inflammatory response syndrome treated by different doses of dexamethasone
10.3760/cma.j.issn.1673-4912.2014.11.005
- VernacularTitle:不同剂量地塞米松对全身炎症反应综合征新生小鼠血清白细胞介素-8、白细胞介素-4、肿瘤坏死因子-α表达的影响
- Author:
Jing YU
;
Wen YANG
;
Ruofei GAO
- Publication Type:Journal Article
- Keywords:
Systemic inflammatory response syndrome;
Dexamethasone;
Cytokine
- From:
Chinese Pediatric Emergency Medicine
2014;21(11):697-699
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression levels of IL-8,IL-4,TNF-α of neonatal mouse with systemic inflammatory response syndrome(SIRS) treated by different doses of dexamethasone.Methods A total of 50 neonatal mice were randomly divided into five groups:blank control group,saline control group and treatment group A,B,C.The saline control group and treatment groups were established into SIRS models,treatment group A:a single high-dose of dexamethasone (2 mg/kg),subcutaneous injection (SC) ; treatment group B:two doses of dexamethasone (1 mg/kg,q1 2 h,total 2 mg/kg,SC) ; treatment group C:four doses of dexamethasone (0.5 mg/kg,q 1 2 h,total 2 mg/kg,SC) ;saline control group:saline of the same volume (0.4 ml/kg,SC).All mice were detected IL-4,IL-8,TNF-α by ELISA 72 hours after animal models being completed.Results The levels of IL-4,IL-8,TNF-α in saline control group and treatment group A,B,C were higher than those in blank control group respectively (P ＜ 0.05,respectively).The levels of IL-4,IL-8,TNF-α in treatment group A,B,C were lower compared to those of saline control group respectively (P ＜ 0.05,respectively),levels of TNF-α,IL-8 in treatment group B and C were lower than those of treatment group A(P ＜ 0.05,respectively).There were no significant differences in the level of IL-4 among treatment group A,B,and C (P ＞ 0.05).Conclusion Dexamethasone could lower the expressions of pro-inflammatory cytokines IL-8,TNF-α and anti-inflammatory factor IL-8 of neonatal mouse with SIRS,and the effect of multiple low-doses of dexamethasone on SIRS is significantly better than a single high dose.
