Periosteum-Derived Mesenchymal Stem Cells and Scaffolds Transplanted into Long-Bone Defects of Rabbit.
- Author:
Hui Taek KIM
1
;
Dong Joon KANG
;
Jeong Han KANG
;
Jong Seo LEE
;
Hee Kyung CHANG
;
Soon Mi PARK
;
Chong Il YOO
Author Information
1. Department of Orthopaedic Surgery, College of Medicine, Pusan National University, Busan, Korea. kimht@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Bone formation;
Mesenchymal stem cell;
Scaffold
- MeSH:
Agar;
Heterografts;
Mesenchymal Stromal Cells*;
Osteogenesis;
Polymers;
Rabbits
- From:Journal of Korean Orthopaedic Research Society
2006;9(2):116-123
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To observe the changes in periosteum-derived mesenchymal stem cells and different scaffold materials transplanted into rabbit long-bone defects. MATERIALS AND METHODS: Thirty-five white rabbits were grouped according to the material transplanted into their tibial bone defects: Group 1 (control group); Group 2-A (agar plus mesenchymal stem cells); Group 2-B (agar only); Group 3-A (Biocompatible Osteoconductive Polymer plus mesenchymal stem cells); Group 3-B (BOP(R) only); Group 4-A (xenograft plus mesenchymal stem cells); Group 4-B (xenograft only). After surgery, radiologic and microscopic observation were performed weekly for 8 weeks. RESULTS: Rabbits transplanted with mesenchymal stem cells showed better bone formation than those without. Mesenchymal stem cells transplanted with agar had better results than mesenchymal stem cells plus BOP(R). Rabbits receiving xenografts showed severe inflammatory reactions. CONCLUSION: Further research is needed on rigid scaffold materials which minimize immune reaction, and on how to ensure uniform distribution of mesenchymal stem cells within such materials.
