Nineteen Types of WNT Genes Mediate The Differentiation of C3H10T1/2 Cell Lines.
- Author:
Sung Keun KIM
1
;
Seung Hye CHOI
;
Sang Seob YUN
;
Won Kyung KANG
;
Hyeon Min CHO
;
Chang Hyuk AHN
;
Yun Seok LEE
;
In Kyu LEE
;
Sang Cheol LEE
;
Youn Jung HEO
;
Do Sang LEE
;
Jong Kyung PARK
;
Seong LEE
Author Information
1. Department of Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea. jkpark@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
WNT genes;
Mesenchymal stem cells;
Osteogenic activity;
Osteblast differentiation
- MeSH:
Adenoviridae;
Alkaline Phosphatase;
Cell Line*;
Humans;
Luciferases;
Mesenchymal Stromal Cells;
Osteoblasts;
Stem Cells
- From:Journal of Korean Orthopaedic Research Society
2006;9(2):124-134
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: In humans, nineteen types of WNT genes (WNTs) have been hightlighted up to date. The canonical Wnt cascade has recently emerged as a critical regulator of stem cells. To obtain new insights how nineteen WNTs affect mesenchymal stem cells differentiation, we analyzed the transcriptional activity, osteogenic and adipogenic activity of WNTs in mesenchymal stem cells. MATERIALS AND METHODS: Recombinant adenoviruses expressing nineteen WNTs were constructed to infect pluripotent mesenchymal progenitor C3H10T1/2 cells. Transcriptional activity was determined by using the luciferase reporter assay. Osteogenic activity was determined by measuring the induction of alkaline phosphatase upon Wnt stimulation. Adipogenic activity was measured by histochemical Oil red-O staining. RESULTS: WNT1, 2, 3, 3A and 10B significantly induced transcriptional activity in C3H10T1/2 cells. WNT1, 2, 3, 3A and 10B significantly induced alkaline phosphatase activity, but inhibited adipogenic activity in C3H10T1/2 cells. The results of qualitative and quantitative assay of alkaline phosphatase activity were consistent with those of luciferase assay for transcriptional activity and Oil red-O staining for adipogenic activity. CONCLUSION: We could expect that WNT1, 2, 3, 3A and 10B may play a crucial role in inducing osteoblast differentiation of mesenchymal stem cells.
