The mRNA expression and clinical significance of ORMDL3 gene in the peripheral blood of recurrent wheeze children
10.3760/cma.j.issn.2095-428X.2014.21.006
- VernacularTitle:反复喘息患儿外周血血清类黏蛋白1样蛋白3基因表达水平的意义
- Author:
Rui JIN
;
Lingzhi QIU
;
Wenxiao YUAN
;
Lili ZHUANG
;
Yuhua HU
;
Guoping ZHOU
- Publication Type:Journal Article
- Keywords:
Orosomucoid 1-like protein 3;
Recurrent wheeze;
Atopy
- From:
Chinese Journal of Applied Clinical Pediatrics
2014;29(21):1618-1620
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mRNA expression levels and clinical significance of omsomucoid 1-like protein 3 (ORMDL3) gene in the peripheral blood of recurrent wheeze children under 3 years old.Methods Peripheral blood specimens of 25 recurrent wheeze children including 14 non-atopy patients (group A) and 11 atopy patients (group B) that were registered in the First Affiliated Hospital of Nanjing Medical University,from Sep.2010 to Sep.2012 were enrolled based on the inclusion criteria and 24 non-allergic controls(the children with food allergy,drug allergy or ectema was excepted).The mRNA expression levels of ORMDL3 gene were detected by reverse transcription (RT)-PCR and clinical features were analyzed.Results The expression levels of ORMDL3 were up-regulated in the peripheral blood specimens of group B compared with group A (t =14.12,P ＜ 0.01).Compared with peripheral blood specimens from normal subjects,mRNA expression of ORMDL3 were significantly increased in recurrent wheeze children(t =10.29,5.73,P ＜0.01).The incidence of wheeze groups exist gender differences,male ＞ female.Wheeze usually with a high incidence in winter and spring.Conclusions The increase of ORMDL3 gene expression levels were correlated with recurrent wheeze under 3 years old especially in atopy group and may be involved in the pathogenesis of recurrent wheeze.
