miR-34c suppresses cell proliferation and invasion of prostate cancer PC3 cells
10.3760/cma.j.issn.1008-1372.2014.09.005
- VernacularTitle:miRNA-34c对人前列腺癌PC3细胞增殖和侵袭能力的影响
- Author:
Hao LIU
;
Jiang YIN
;
Zhimin HE
- Publication Type:Journal Article
- Keywords:
MicroRNAs;
Prostatic neoplasms/pathology;
Prostatic neoplasms/genetics;
Tumor cells,cultured;
Cell proliferation;
Neoplasm invasiveness;
Polymerase chain reaction
- From:
Journal of Chinese Physician
2014;16(9):1168-1171
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect and molecular mechanism of miR-34c in cell proliferation and invasion of human prostate cancer PC3 cells.Methods miR-34c was synthesized and transfected into prostate cancer PC3 cells.The ability of cell pro liferation was examined with methyl thiazolyl tetrazolium (MTT),and invasion with Transwell assay.The level of c-met mRNA was analyzed by real-time reversing transcription polymerase chain reaction (RT-PCR).Western blot was used to detect the expressions of cmet,cyclin D1,and EMT-associated markers.Results Over-expression of miR-34c reduced PC3 cell proliferation,and inhibited the abilities of invasion.Bioinformatics analysis showed that 3'-UTR of c-met had two miR-34c matched sites,and miR-34c inhibited the expressions of c-met mRNA and protein.Meantime,miR-34c obviously decreased the expressions of cyclin D1 and N-cadherin,but increased the expression of E-cadherin in PC3 cells.Conclusions miR-34c may suppress cell proliferation and invasion in prostate cancer PC3 cells,by which mechanism is related to reduction of the expressions of c-met,cyclin D1,N-cadherin,and upregulation of E-cadherin.
