Difference in HLA-DR Expression of Human Umbilical Cord Blood Derived Mesenchymal Stem Cells after Tri-lineage Differentiation.
- Author:
Geum Ok BAEK
1
,
2
;
Ji Hye SUNG
;
Jeong Seok MOON
;
Chul Won HA
Author Information
1. Department of Orthopedic Surgery, Samsung Medical Center, SungkyunKwan University, School of Medicine, Seoul
2. Medipost Biomedical Research Institute, Yongin, Korea. hacw@smc.samsung.co.kr
- Publication Type:Original Article
- Keywords:
Human umbilical cord blood;
Mesenchymal stem cells;
Chondrogenic differentiation;
Immunologic properties;
HLA-DR
- MeSH:
Antigens, Surface;
Bone Marrow;
Chondrocytes;
Fetal Blood*;
HLA-DR Antigens*;
Humans*;
Mesenchymal Stromal Cells*;
Umbilical Cord*
- From:Journal of Korean Orthopaedic Research Society
2006;9(2):188-194
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The aim of this study was to examine the expression of HLA-DR surface antigen in undifferentiated human umbilical cord blood (hUCB) derived mesenchymal stem cells (MSCs) and after osteogenic, chondrogenic, and adipogenic differentiation. MATERIALS AND METHODS: hUCB-derived MSCs were differentiated into osteogenic, chondrogenic, and adipogenic lineages. Differentiation was assessed by immunohistochemical staining and RT-PCR. The expression of HLA-DR was assessed with antihuman HLA-DR antibody in undifferentiated hUCB-derived MSCs and after tri-lineage differentiation. RESULTS: HLA-DR expression was negative in undifferentiated hUCB-derived MSCs and after osteogenic and adipogenic differentiation. However, HLA-DR surface antigen was expressed after chondrogenic differentiation. CONCLUSION: The immunologic properties of hUCB-derived MSCs differ from known reports on bone marrow derived MSCs from the results of this study. Careful immunological survey seems to be needed in case of considering the transplantation of hUCB-derived MSCs differentiated into chondrocytes or cartilaginous tissue.
