Experimental study on the differentiation of SHI-1 cells induced by puerariae radix flavones in vitro

VernacularTitle:葛根总黄酮体外诱导急性单核细胞白血病SHI-1细胞分化的实验研究
Author: Guohua ZHU ; Qi ZHANG ; Haiping DAI ; Yunliang ZHAI ; Qun SHEN
Publication Type:Journal Article
Keywords: Pueranae radix flavones; Cells,SHI-1; Cell differentiation
From: Journal of Leukemia & Lymphoma 2013;22(10):582-585
CountryChina
Language:Chinese
Abstract: Objective To explore the possible effects on differentiation of SHI-1 cells induced by puerariae radix flavones(PRF)in vitro.Methods SHI-1 cells were treated with PRF in various concertration,then the inhibitory effects of cell proliferation were detected by MTT assay,the cell cycles were analyzed by flow cytometry(FCM),the cells reduction rates were detected by NBT reduction test,and the expression of CD11b and CD14 were tested by FCM.Results 10-50 μg/ml PRF could inhibit the proliferation of SHI-1 cells in a time-and dose-dependent manner,and the cell cycles were arrested in S phase.When SHI-1 cells were treated with 10,30 and 50 μg/ml PRF in 48 houres respectively,the NBT reduction rates of cells were increased in a dose-dependent with PRF(P＜0.05),and the expression of cells surface differentiation antigen CD14 was also increased along with the concentration of PRF.Conclusion The SHI-1 cells could be induced to differentiation partially after treated with 10,30 and 50 μg/ml PRF in vitro.