Effect of curcumin microemulsion on proliferation and apoptosis of human leukemia cell line K562 cells
10.3760/cma.j.issn.1009-9921.2013.10.002
- VernacularTitle:姜黄素微乳对人类白血病K562细胞增殖和凋亡的影响
- Author:
Hongyan WU
;
Lingling GENG
;
Zhigang LIU
;
Yalin DONG
;
Min HE
- Publication Type:Journal Article
- Keywords:
Curcumin;
Microemulsion;
Cell proliferation;
Apoptosis;
Gene,WT1
- From:
Journal of Leukemia & Lymphoma
2013;22(10):578-581
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effects of curcumin microemulsion on proliferation and apoptosis of the human leukemia cell line K562 cells.Methods The curcumin microemulsion was prepared with the routine procedure.MTT assay was used to determine cell proliferation in cultured K562 cells,flow cytometry analysis was applied to examine cell apoptosis,and WT1 mRNA was determined with RT-PCR.The results about curcumin microemulsion were compared with these on curcumin.Results The prepared curcumin microemulsion was a stable clear solution with diameter of 10-100 nm.Curcumin microemulsion inhibited K562 cell proliferation by 24％,46％,68％with a 24 h incubation at dose of 2.5 μg/ml,5.0 μg/ml,and 10.0 μg/ml respectively,whereas curcumin reduced the proliferation by 6％,14％,25％at equal concentrations.WT1 mRNA level of curcumin microemulsion group(0.190±0.036)was reduced stronger than that of curcusin group(0.456±0.047).Conclusions Microemulsion is a great carrier for curcumin.Curcumin microemulsion is more effective in inhibiting proliferation,pro-apoptosis,and reducing WT1 gene expression than curcumin.A strong basis of medical value for the use of curcumin microemulsion to treat tumors is provided.
