Expression of T cell immunogloblin and mucin domain-containing molecule 3 mRNA and its relationship with asthma
10.3760/cma.j.issn.2095-428X.2014.21.005
- VernacularTitle:T淋巴细胞免疫球蛋白黏蛋白-3 mRNA表达在支气管哮喘中的作用
- Author:
Feng HAN
;
Xiaoxia LU
;
Ying WANG
- Publication Type:Journal Article
- Keywords:
Asthma;
T cell immunogloblin and mucin domain-containing molecule 3;
T lymphocyte
- From:
Chinese Journal of Applied Clinical Pediatrics
2014;29(21):1614-1617
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of T cell immunogloblin and mucin domain-containing molecule 3 (Tim-3) mRNA in peripheral blood mononuclear cells (PBMC) isolated from asthmatic children and asthmatic murine,and to explore the role of Tim-3 in bronchial asthma.Methods Recruiting 97 cases asthmatic children,47 children with acute asthma exacerbation(acute attack group),50 children with asthma remission (remittent group)and 42 children as the healthy control group.The Tim-3 mRNA expression was measured by using reverse transcription-polymerase chain reaction(RT-PCR) method.The levels of interleukin-6 (IL-6) and transforming growth factor-5 (TGF-β)were determined with enzyme-linked immunosorbent assay (ELISA),and to analyze the correlation between Tim-3 mRNA and the level of IL-6.The asthmatic murine models were randomly divided into 2 groups:normal control group and asthma group.There were 10 mice in each group.PBMC in each group were collected.Expression of Tim-3 mRNA was detected by using real-time PCR.The level of CD4 + IL-17 + and CD4 + CD~ + FoxP3 + which reflected Th17 and regulatory T cells(Treg) expression respectively were detected by using flow cytometry,and the correlation between Tim-3 and Thl7/Treg level was analyzed.Results Expression of Tim-3 mRNA in children with acute asthma exacerbation was significantly higher than those in remission stage and healthy control group (all P ＜ 0.05).The expression of Tim-3 mRNA in children with asthma remission was significantly higher than that in healthy control group (P ＜ 0.05),and the level of IL-6 in children with acute asthma exacerbation was significantly higher than those in remission stage and healthy control group (all P ＜ 0.05).In the murine model of asthma group,the level of Tim-3 mRNA in PBMC was significantly higher than that in normal control group (P ＜ 0.01),and in asthma group CD4 + IL-17 + expression was significantly higher than normal control group but CD4 + CD25 + FoxP3 + was significantly lower than that in normal control group (all P ＜ 0.05),and the ratio of Th17/Treg increased in asthma group compared with normal control group (P ＜ 0.05).Tim-3 mRNA expression in PBMC was correlated positively with CD4 + IL-17 + level and Th17/Treg ratio respectively (all P ＜ 0.05),but was correlated negatively with CD4 + CD25 + FoxP3 + level(all P ＜ 0.05).Conclusions The high level expression of Tim-3 mRNA in asthmatic children participated in the airway inflammation,which is associated with IL-6,and animal experiments indicating that Tim-3 is closely related with the imbalance of Th1 7/Treg,indicated that Tim-3 may play a certain role in asthma.
