Expression of FAS and FASL in brain of sodium valproate induced autism rats
10.3760/cma.j.issn.2095-428X.2014.20.016
- VernacularTitle:丙戊酸钠诱导孤独症模型大鼠大脑组织中FAS/FASL表达的意义
- Author:
Zhimei JIANG
;
Jin GUO
;
Yajun WANG
;
Lijun CUI
;
Xin LI
- Publication Type:Journal Article
- Keywords:
Autism;
Sodium valproate;
FAS;
FASL;
Rat
- From:
Chinese Journal of Applied Clinical Pediatrics
2014;29(20):1575-1577
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of FAS/FASL in brain of autism model rats induced by sodium valproate,and study the role of FAS/FASL in pathogenesis of autism.Methods Female wistar rats were given a single intraperitoneal injection of sodium valproate(VPA,600 mg/kg) on 12.5 d after pregnancy,and their offspring were used as the experimental group;while the other pregnancy rats were given the same dose of normal saline,and their offspring were used as the control group.The growth,social and non-social behaviors,repetitive stereotyped movements of both groups were observed and tested.The expressions of FAS/FASL in brain of both groups on 1 d,7 d,l4 d,28 d,56 d and 90 d after birth were observed and compared by means of immunohistochemistry and image analysis.Results Compared with the control group,the rats in experimental group had stunted growth,a decrease in social behavior,an increase in non-social behavior,with repetitive stereotyped movements increased,and there were significant differences (all P ＜0.05) ;FAS/FASL expression was less on 1 d after birth,and it was growing gradually with age in days,and expression reached the peak on 28 d and then began to decrease.Compared with the control group,all day-old rats' FAS and FASL expression in the experimental group were significantly raised (all P ＜ 0.05).In experimental group,the brain FAS/FASL IOD values on 28 d after the birth reached the maximum,and 90 d after the birth reached the minimum.In the control group brain FAS/FASL IOD values on 14 d after the birth reached the maximum,and 1 d after the birth reached the minimum.Conclusions The apoptotic cell of autism model rats' brain neure is increasing firstly and then decreasing by FAS/FASL apoptotic pathway,and reach apoptosis peak on 28d.
