Effects of decitabine on heat-shock protein 22 expression in malignant hematopoietic cells
10.3760/cma.j.issn.1009-9921.2013.05.003
- VernacularTitle:地西他滨对造血系统肿瘤细胞热休克蛋白22表达的影响
- Author:
Lina WANG
;
Xueying CUI
;
Xiaotong MA
;
Qian REN
;
Nan WANG
- Publication Type:Journal Article
- Keywords:
Hematologic neoplasms;
Decitabine;
Heat-shock protein 22
- From:
Journal of Leukemia & Lymphoma
2013;22(5):263-266
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and possible mechanisms of decitabine on heat-shock protein 22 (HSP22) expression in hematopoietic tumor cell lines and bone marrow samples from patients with hematopoietic tumor.Methods The expression of HSP22 in 13 hematopoietic tumor cell lines,20 primary patients' samples and 10 normal donor' samples were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).After HSP22 induction with a demethylating agent decitabine (2 μmol/L),the methylation of the HSP22 promoters in hematopoietic tumor cell lines,healthy donors and bone marrow samples from patients with hematopoietic tumor were detected by methylation specific PCR (MSP).Results Expression of HSP22 was not detected in 13 hematopoietic tumor cell lines,20 primary patients' samples or 10 healthy donors' samples.Decitabine can induce the expression of HSP22 in hematopoietic tumor cell lines and bone marrow samples from patients with hematopoietic tumor.Decitabine can maintain partially demethylation of HSP22 promoters in hematopoietic tumor cell lines.HSP22 promoters were highly methylated in BMMC of the healthy donors and patients with hcmatopoictic tumor.Conclusion Decitabine can induce the expression of HSP22 in hematopoietic tumor cells partly by demethylation of HSP22 promoters.
