Effect of CCAAT/enhancer-binding protein homologeus protein on differentiation in Tan Ⅱ A induced acute promyelocytic leukemia cells
10.3760/cma.j.issn.1009-9921.2013.03.006
- VernacularTitle:CCAAT增强子结合蛋白同源蛋白在丹参酮ⅡA诱导急性早幼粒细胞白血病细胞分化中的作用
- Author:
Kaiji ZHANG
;
Jishi WANG
;
Jian LI
;
Jinzhu CAO
;
Yiming YANG
- Publication Type:Journal Article
- Keywords:
Tan Ⅱ A;
CHOP;
Leukemia,promyelocytic,acute;
Differentiation;
Apoptosis
- From:
Journal of Leukemia & Lymphoma
2013;22(3):147-150
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of CCAAT/enhancer-binding protein homologeus protein (CHOP) in Tan Ⅱ A treated acute promyelocytic leukemia (APL) cells.Methods APL cell differentiation was monitored by morphology and membrane differentiation antigens; expression of CHOP was inhibited by siCHOP; mRNA and protein expression of CHOP in Tan Ⅱ A-intervened APL cells were examined by RT-PCR and Western blot.Results Expression of CHOP was increased in Tan Ⅱ A induced differentiated APL cells (proteins levels 1.933±0.987 vs 0.537±0.110,F =114.852,P < 0.01,mNRA levels 1.587±0.815 vs 0.713±0.090,F =52.256,P < 0.01),combination of CHOP gene silencing with Tan Ⅱ A treatment induced strong APL cell differentiation [(50.767±1.241) % vs (16.167±2.122) %,F =989.431,P < 0.05] and apoptosis [(89.233±5.581) % vs (27.433±2.957) %,F =308.961,P < 0.05].Conclusion CHOP acts as a negative regulator in Tan Ⅱ A induced differentiation.Inhibition of CHOP may be a promising therapeutic strategy.