Expression of MT1, 2, 3-MMP in invasive cervical cancer.
- Author:
Hye Sung MOON
1
;
Soon Hee SUNG
;
Hye Won CHUNG
;
Seoung Cheol KIM
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Ewha Womans' University, Seoul, Korea. mhsmhs@ewha.ac.kr
- Publication Type:Original Article
- Keywords:
MT-MMPs;
Cervical cancer
- MeSH:
Blotting, Western;
Cervix Uteri;
Female;
Humans;
Lymph Nodes;
Matrix Metalloproteinase 14;
Matrix Metalloproteinase 16;
Matrix Metalloproteinases;
Neoplasm Metastasis;
Peptide Hydrolases;
Uterine Cervical Neoplasms*
- From:Korean Journal of Obstetrics and Gynecology
2006;49(6):1240-1248
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Threre are several proteolytic enzymes such as Matrix Metalloproteinases (MMP), which are involved in tumor invasion and metastasis. The aim of this study was to determine the exprssion of Membranous Type MMPs (MT-MMPs) and investigate the relationship between their expression and questioned whether their expression is related to stages and other prognostic factors of cervical cancer. METHODS: The cervical and cervical cancer tissues were taken from the patients; healthy women (n=14), and the patients with cervical cancer (n=35). The protein expression of MT1, 2, 3-MMP with MMP-2 was examined using immunohistochemical staining and western blotting. MMP-2 activity was measured by zymogram. RESULTS: The expression of MT1, 2, 3-MMP was higher in cervical cancer than that of normal cervix (p<0.05). No significant association was found between MT-MMPs and clinicopathologic factors, such as age, grade, stage, tumor sizes, and Squamous cell carcinoma-Ag (SCC-Ag) (p>0.05). But there were significant correlations between MT1-MMP, MT3-MMP and lymph node involvemen t (p<0.05). There was significant correlation between MT-MMPs and MMP-2 (p<0.05), too. CONCLUSION: According to the results, MT-MMP expression could be associated with the pathogenesis of cervical cancer. In addition, the evaluation fo MT-MMPs expression might be helpful to predict lymph node metastasis in cervical cancer. Further prospective study with a large number of cases is needed in future.
