Correlation between TNF-α-238 gene polymorphism and hepatitis B virus reactivation in patients with malignant tumors after chemotherapy
10.3760/cma.j.issn.0254-9026.2015.07.017
- VernacularTitle:肿瘤坏死因子-α-238基因多态性与肿瘤化疗后乙型肝炎病毒再激活的关系
- Author:
Derong LU
;
Shiyu GU
;
Cailing JIN
;
Shupeng ZHAO
- Publication Type:Journal Article
- Keywords:
Tumor necrosis factor alpha;
Hepatitis B virus;
Virus activation
- From:
Chinese Journal of Geriatrics
2015;34(7):756-759
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between the single nucleotide polymorphism (SNP) of tumor necrosis factor (TNF-α) gene promotor-238 and hepatitis B virus (HBV) reactivation in patients with malignant tumors after chemotherapy.Methods Polymerase chain reaction-restriction fragment length polymorphism assay (PCR-RFLP) was used to detect the SNPat TNF-α-238 site among 100 malignant tumor patients with HBV infection.HBV-DNA levels in patients were detected before and after chemotherapy.HBV reactivation was defined as that HBV-DNA level greater than 10-fold increase compared with before chemotherapy or higher than 1 × 109 logcopies/ml.Results The quantification of HBV-DNA was higher after chemotherapy than before chemotherapy [(3.02±0.68) logcopies/ml vs.(2.49±0.23) logcopies/ml,t=-7.383,P=0.000].Among the patients with malignant tumor and HBV infection,the genotype frequency of G/A was higher in HBV reactivation group than in non-reactivation group after chemotherapy [27.3％ (6/22) vs.3.8％ (3/ 78),x2 =11.499,P=0.001].Conclusions HBV reactivation is associated with TNF-α-238 gene polymorphism in malignant tumor patients with HBV infection after chemotherapy.
