Attenuated store-operated Ca2+ entry in mesenteric artery endothelial cells of aged rat
10.3760/cma.j.issn.0254-9026.2015.04.025
- VernacularTitle:衰老对大鼠肠系膜上动脉内皮细胞钙库操纵钙内流的影响
- Author:
Xia WANG
;
Jie LI
;
Yan ZHU
;
Juan DU
;
Bing SHEN
- Publication Type:Journal Article
- Keywords:
Mesenteric artery superior;
Endothelial cells;
Calcium
- From:
Chinese Journal of Geriatrics
2015;34(4):434-437
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe attenuated store-operated Ca2+ entry in superior mesenteric artery endothelial cells of aged rat.Methods The pressure myograph was applied to measure the vasodilation of superior mesenteric artery induced by bradykinin (BK) in aged versus non-elder rats.Primarily cultured MAECs were treated by thapsigargin (TG) and BK to deplete intracellular Ca2+ stores for comparing SOCE.SOCE of MAECs was detected by calcium imaging technology and was compared between aged and non-elder rats.The expression levels of Orai 1 and stromal interaction factor 1 (STIM 1) were observed by immunofluorescence method.Results Compared with the nonelder rats (70.0 ± 4.1 ％),BK-induced vasodilation in aged rats (27.0 ± 4.9)％ was declined by (60.7±4.3) ％.SOCE induced by BK and TG in primarily cultured MAECs was attenuated by 37.6％ and 39.2％ in aged rats (1.71±0.18 and 1.06±0.03) respectively as compared with non-elder rats (2.72±0.39 and 1.75±0.06).The expressions of SOCE's components Orai 1 and STIM 1 in MAECs were decreased obviously in aged rats than in non elder rats.Conclusions SOCE of MAECs and SOCE-induced vasodilation are significantly attenuated in aged rats.The decreased expression level of Orai 1 and STIM 1 may contribute to this alteration.
