The establishment and application of a time-resolved fluoroimmunoassay in detection of HBV large surface protein
10.3760/cma.j.issn.2095-2848.2014.05.006
- VernacularTitle:乙型肝炎病毒大蛋白时间分辨荧光免疫分析法的建立及应用
- Author:
Mei LI
;
Hualong XIAO
;
Jie LIU
;
Zhigang HU
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Viral envelope proteins;
Fluoroimmunoassay
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2014;34(5):362-365
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method of TRFIA with high sensitivity and broad detecting range for serum HBV large surface protein (HBV-LP).Methods The monoclonal antibody of HBV-LP was covered on the microwell plate and incubated with HBV-LP in blood sample,then Eu3+ labeled antibody of HBs was added.HBV-LP in standard substance,blood samples of 66 chronic hepatitis B patients and 30 healthy controls was detected by the TRFIA and ELISA.x2 test and linear correlation analysis were used for data analysis.Results The dose-response curve of standard substance with TRFIA had good linear correlation (r=0.999).Normal reference range was established at 0-1.36 mg/L based on the ELISA results of 30 healthy controls.The sensitivity was 0.10 mg/L.The specificity was 100% (30/30).Correlation coefficient between the TRFIA and the ELISA was 0.800 9 (P<0.001).The positive detecting rates of the 2 methods were significantly different (89.4%(59/66) vs 77.3%(51/66),x2 =6.13,P<0.01).The recovery rate for HBV-LP was between 95.93%-107.62%.The effective detecting range(CV<10%) of TRFIA was 1.35-2 764.00 mg/L,and that of ELISA was 10.8-691.0 mg/L.Conclusion The TRFIA was established for HBV-LP detection with higher sensitivity and wider detecting range compared to ELISA.It has potential value for HBV screening and monitoring of antiviral therapy.